Radioimmunological determination of serum 3 beta-hydroxy-5-cholenoic acid in normal subjects and patients with liver disease.

Radioimmunological determination of serum 3 beta-hydroxy-5-cholenoic acid in normal subjects and patients with liver disease.

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A radioimmunoassay for the determination of 3 beta-hydroxy-5-cholenoic acid in human serum has been developed, using 3 beta-hydroxy-5-cholenoyl-thyroglobulin as immunogen and 3 beta-hydroxy-5-cholenoylglycyl-125I histamine as radioactive ligand.The association constant was 6.3 X 10(8) l/mol.Cross reactivity with other bile acids of human serum was not detectable, but was 5.

6% with cholesterol.Serum sample preparation included extraction of 3 beta-hydroxy-5-cholenoic acid from serum, solvolysis of sulfates, hydrolysis of conjugates, and separation from cholesterol by thin-layer Neff I96BMV5N5B 90cm Stainless Steel Island Cooker Hood chromatography.Serum concentrations of 3 beta-hydroxy-5-cholenoic acid were 0.23 +/- SD 0.

12 mumol/l and 0.21 +/- SD 0.09 mumol/l in healthy males and females, respectively.In patients with primary biliary cirrhosis the serum concentration of 3 beta-hydroxy-5-cholenoic acid and the quotient 3 beta-hydroxy-5-cholenoic acid over total 3 alpha-hydroxy-bile acids (measured enzymatically) were significantly higher (P less than 0.

02) than in patients with KYOLIC FORMULA 104 chronic active hepatitis or alcoholic cirrhosis.Analysis of 17 sera with elevated concentration of 3 beta-hydroxy-5-cholenoic acid by radioimmunoassay and capillary gas-liquid chromatography showed a close correlation (r = 0.91, slope = 0.97) between the results of the two methods.